HPLC Analysis of Caffeoylquinic Acids in the Extract of Cacalia firma and Peroxynitrite Scavenging Effect
2009
Park, H.J., Sangji University, Wonju, Republic of Korea | Nugroho, Agung, Sangji University, Wonju, Republic of Korea | Lee, J.H., Kangwon National University, Chuncheon, Republic of Korea | Kim, J.D., Kangwon National University, Chuncheon, Republic of Korea | Kim, W.B., Highland Agriculture Research Center, RDA, Pyeongchang, Republic of Korea | Lee, K.R., Sungkyunkwan University, Suwon, Republic of Korea | Choi, J.S., Pukyong National University, Busan, Republic of Korea
Six caffeoylquinic acids of Cacalia firma (Komarov) Nakai (Compositae) leaves were identified using standard compounds by HPLC. Each content of those compounds in dried weight was determined as follows: 3,4-di-O-caffeoylquinic acid (1.44±0.04 mg/g of dried weight), 3,5-di-O-caffeoyl-muce-quinic acid (2.47±0.12 mg/g), 3,5-di-O-caffeoylquinic acid (3.74±0.24 mg/g), 5-caffeoylquinic acid (chlorogenic acid, 5.20±0.09 mg/g), 3-caffeoylquinic acid (1.35±0.01 mg/g) and 3-Op-coumaroylquinic acid (3.84±0.25 mg/g). The total content of six caffeoylquinic acids in the plant material was calculated as 18.05±0.69 mg/g while the percentage of the six compounds in the MeOH extract was calculated as 30.85±1.18%. The IC∧50 value of the MeOH extract scavenging peroxynitrite (ONOO-) was shown as 3.22±0.57 ㎍/ml.
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