Plant regeneration from callus derived root of northen type in garlic (Allium sativum L.)
2009
Ahn, Y.K., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea | Kim, D.S., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea | Yoon, M.K., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea
This study was conducted to develop an effective production of callus induction and plant regeneration system for garlic transformation. The best callus production occurred on in vitro root segment initially cultured on MS medium with 1.0 mg/L 2,4-D and 0.2 mg/L IAA in both 'Danyang' and 'Euseong'. The frequency of callus formation were 81.2% 'Danyang' and 76.1% 'Euseong'. Eight weeks after callus induction, callus lines were transferred to regeneration medium during 7 weeks. The best shoot regeneration medium was MS supplemented with 5 mg/L Kinetin and 1 mg/L NAA for 'Danyang' and MS supplemented with 10 mg/L BAP for 'Euseong'. The frequency of shoot regeneration were 51.5% 'Danyang' and 56.6% 'Euseong' The plantlets were acclimatized and transferred to the greenhouse with almost survival. This in vitro regeneration system should be useful for garlic transformation.
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