Biochemical activities of bacterial species isolated from the frozen semen of cattle
2009
Abro, S.H. (Sindh Agriculture Univ., Tandojam (Pakistan). Dept. of Microbiology) | Wagan, R. (Sindh Agriculture Univ., Tandojam (Pakistan). Dept. of Microbiology) | Tunio, M.T. (Allama Iqbal Open Univ., Islamabad (Pakistan). Dept. of Agricultural Sciences) | Kamboh, A.A. (Sindh Agriculture Univ., Tandojam (Pakistan). Dept. of Microbiology) | Munir, M. (SLU Uppsala (Sweden). Dept. of Biomedical Sciences and Veterinary Public Health)
In order to study the bacterial contamination of frozen semen of cattle, 100 frozen semen samples collected from Semen Production Centers of Karachi and Rohri were analyzed. Biochemical and sugar fermentation tests were carried-out to confirm the specific characteristics of each bacterial isolate and to differentiate between various species of the same genus. Different species of bacteria isolated from the frozen semen samples were Acinetobacter, Actinobacillus lignierisis, Citrobacter, Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus epidermidis and Staphylococcus intermedius. Actinobacillus ligneirisi showed the positive to catalase, methyl red, methyl blue and urease, while negative to coagulase, methyl red, aesculin, Voges Proskauer and oxidase. Acinetobacter species exhibited high reactivity of catalase and urease enzymes. Citrobacter species showed negative reaction to indole and oxidase-negative and non-fermenters of mannitol, raffinose and lactose. Micrococcus luteus was showed variable reaction in Hugh and Leifson's medium and failed to degrade urea. Pseudomonas aeruginosa reacted positively to catalase, oxidase and gelatin liquefaction. While, negative for methyl blue, methyl red, Voges Proskauer, indole, hydrogen peroxide and hydrogen sulfide. Staphylococcus intermedius fermented mannitol, while Staphylococcus epidermidis did not. Gelatin was liquefied by Staphylococcus intermedius, while Staphylococcus epidermidis did not liquefy gelatin disc. Staphylococcus epidermidis was urease positive, while Staphylococcus intermedius was urease negative.
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