In vitro maturation and germination of Jatropha curcas microspores
2010
Li, C. (Sichuan Univ., Chengdu (Republic of China). Ministry of Education) | Yu, M. (Sichuan Univ., Chengdu (Republic of China). Ministry of Education) | Chen, F. (Sichuan Univ., Chengdu (Republic of China). Ministry of Education) | Wang, S.
In the present study, we established an experimental system of in vitro maturation and germination of Jatropha curcas L. microspore. Our results suggested that the most appropriate medium for in vitro culture of J. curcas microspores was MS salts + White' vitamins + 22% maltose, pH6.5 and the maturation and germination rate were 39.5% and 2.7%, respectively. Liquid medium with 20% sucrose+10% PEG-4000+BK [Ca (NO3) 2 400 mg/L, MgSO4 200 mg/L, H2BO3 100 mg/L, KNO3 100 mg/L] + 2.5% coconut water, pH6.5 was optimal for germination of pollen matured in vivo. The rate of germination was 71.6%. Pollen matured in vitro exhibited a rate of germination of 13.7%, when it was transferred into the optimal medium for pollen germination.
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