Koi herpes virus: do acipenserid restitution programs pose a threat to carp farms in the disease-free zones?
2009
Kempter, J. | Sadowski, J.,West Pomeranian University of Technology, Szczecin (Poland). Dept of Aquauclture | Schuetze, H. | Fischer, U. | Dauber, M. | Fichtner, D.,Federal Research Institute for Animal Health, Suedufer (Germany) | Panicz, R.,West Pomeranian University of Technology, Szczecin (Poland). Dept of Aquauclture | Bergmann, S.M.,Federal Research Institute for Animal Health, Suedufer (Germany)
Total of 29 sturgeons (two species; length 8-37 cm) originating from fish farms in northern Poland with a known KHV history in common carp or koi in the area were examined: 15 Russian sturgeons, Acipenser gueldenstaedtii, with clinical signs of a disease and 14 asymptomatic Atlantic sturgeons, A. oxyrinchus. The preliminary results were obtained using PCR. Those findings were subsequently confirmation by nested PCR. The latter procedure consists of sequence analysis of PCR products and direct detection of KHV infected cells in tissue materials by in-situ hybridization on nucleic acid level or indirect immunofluorescence on KHV protein level. KHV genome parts were found in nine Russian sturgeons and four Atlantic sturgeons. Therefore a viral diagnostics is highly recommended not only for sturgeons obtained from the environment but also for fertilized eggs, fry, and fish intended for re-stocking measurements of inland waters
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