Influence of gamma-radiation on antioxidant system of selected genotypes of soybean and oxidative stress appearance. [Doctoral dossertation] | Uticaj gama-zracenja na antioksidativni sistem odabranih genotipova soje i pojava oksidativnog stresa. Doktorska disertacija
2006
Popovic, B.
The results obtained through the investigation about the influence of low level doses of gamma-radiation (up to 200 Gy) on the antioxidative properties and oxidative stress parameters in different phases of soy development and the influence of the middle level doses of gamma-radiation (from 1 kGy to 10 kGy) on some antioxidant parameters of soybean seed were presented in this thesis. By comparison of antioxidant statuses of irradiated and nonirradiated soy, it was established that the gamma-radiation of soybean stimulated the antioxidant system of soy during the development through two generations. It was observed that the changes in antioxidant activities of different components of antioxidant system of soybean depend on dose of radiation, genotype and phase of the development. On the basis of lipid peroxidation as criterion (biomarker) of sensitivity on radiation and oxidative stress, investigated genotypes can be classified into two groups: radiosensitive genotypes Venera and Ana and radioresistant genotypes Meli and Novosadjanka. Results of ESR spectroscopy confirmed that genotypes Ana and Venera are more sensitive to radiation and oxidative stress than genotypes Meli and Novosadjanka. Our results indicate that middle doses of gamma-radiation possess such antioxidative activity which is manifested with the increase of DPPH scavenger activity and decrease of protein oxidation intensity in soybean seed. We did not establish any negative implication on investigated nutritive characteristics of soybean seed.
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