Sulfonylurea tolerance of wheat genotypes in zygotic embryo culture
2009
Kondic-Spika, A. | Petrovic, K. | Jevtic, R. | Kobiljski, B., Institute of Field and Vegetable Crops, Novi Sad (Serbia) | Pucarevic, M., EDUCONS Faculty of Environmental Protection, Sremska Kamenica (Serbia)
Tolerance of wheat-genotypes to the sulfonylurea herbicide metsulfuron-methyl was studied using in vitro culture. Six randomly selected wheat genotypes were used for isolation. Mature embryos were inoculated onta a modified MS medium to which three concentrations of metsulfuron-methyl were added: 1st - 30 g/l, 2nd - 60 g/l, and 3rd - 90 g/l. The control group of embryos was cultivated on a herbicide-free medium. After one month of cultivation, callus fresh weight was measured. There were significant differences among the genotypes regarding their tolerance to metsulfuron-methyl. The Pobeda cultivar expressed the highest and the Lr-12 genotype had the lowest level of herbicide tolerance.
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