Seed-specific aspartic proteinase FeAP12 from buckwheat (Fagopyrum esculentum Moench)
2010
Timotijevic, G.S., University of Belgrade, Belgrade (Serbia). Institute of Molecular Genetics and Genetic Engineering | Milisavljevic, M.Dj., University of Belgrade, Belgrade (Serbia). Institute of Molecular Genetics and Genetic Engineering | Radovic, S.R., University of Belgrade, Belgrade (Serbia). Faculty of Biology | Konstantinovic, M.M., University of Belgrade, Belgrade (Serbia). Institute of Molecular Genetics and Genetic Engineering | Maksimovic, V.R., University of Belgrade, Belgrade (Serbia). Institute of Molecular Genetics and Genetic Engineering
Aspartic proteinase gene (FeAP12) has been isolated from the c DNA library of developing buckwheat seeds. Analysis of its deduced amino acid sequence showed that it resembled the structure and shared high homology with typical plant aspartic proteinases (AP) characterized by the presence of a plant-specific insert (PSI), unique among APs. It was shown that FeAP12 mRNA was not present in the leaves, roots, stems, and flowers, but was seed-specifically expressed. Moreover, the highest levels of FeAP12 expression were observed in the early stages of seed development, therefore suggesting its potential role in nucellar degradation.
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