DTBA and ELISA methods in detection of grapevine leafroll-1 virus
2006
Ivanovic, Z., Institute for Plant Protection and Environment, Belgrade (Serbia) | Kuzmanovic, S., Institute for Plant Protection and Environment, Belgrade (Serbia) | Trkulja, N., Institute for Plant Protection and Environment, Belgrade (Serbia) | Zivkovic, S., Institute for Plant Protection and Environment, Belgrade (Serbia) | Stojanovic, S., Institute for Plant Protection and Environment, Belgrade (Serbia) | Starovic, M., Institute for Plant Protection and Environment, Belgrade (Serbia)
Grapevine leafroll syndrome is caused by a complex of up to nine different Grapevine leafroll-associated viruses (GLRaV-1-9). Many methods, including indexing, serological and molecular procedures, have been developed for the detection of GLRaV. However, due to the low concentration of the virus in plants a method with improved sensitivity and with the capacity to detect is required. In this study plants were tested for Grapevine leafroll virus (GLRaV) by visual examination, enzyme-linked immunosorbent assay (ELISA) and direct tissue-blotting assay (DTBA). Tissues were homogenized for use in a double-antibody sandwich ELISA system and then blotted on a nitrocellulose membrane for DTBA. Comparison of DTBA with ELISA and with visual plant symptoms suggest that DTBA can be used with the same accuracy as ELISA for detecting GLRaV-1 in leaf tissue.
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