Genetic variability of grapevine fanleaf virus (GFLV) and its biological impact | Genska raznovrstnost virusa pahljacavosti listov vinske trte (GFLV) in njen bioloski pomen
2007
Pompe Novak, M., National Inst. of Biology, Ljubljana (Slovenia) | Gutierrez-Aguirre, I. | Vojvoda, J. | Blas, M. | Tomazic, I., Garibaldijeva 1, 6000 Koper (Slovenija) | Korosec-Koruza, Z., Ljubljana Univ. (Slovenia). Biotechnical Fac., Agronomy Dept | Vigne, E., 28, rue de Herrlisheim, 68021 Colmar cedex (France) | Fuchs, M., Geneva, NY 14456(USA) | Ravnikar, M., Ljubljana Univ. (Slovenia). Biotechnical Fac., Agronomy Dept | Petrovic, N., Ljubljana Univ. (Slovenia). Biotechnical Fac., Agronomy Dept
Grapevine fanleaf virus (GFLV) is a causal agent of grapevine fanleaf degeneration disease, which is one of the most important viral diseases of grapevine that results in progressive decline of infected vines, yield loss and poor fruit quality in all wine producing areas in the world. The virus is spread naturally by a nematode vector Xiphinema index and through the use of infected planting material. Classical control measures are the use of healthy planting material and control of the vector. New methods comprise the introduction of transgenic grapevines, resistant to GFLV. Because the resistance is mainly based on expressing GFLV coat protein transgene, the assessment of environmental risks, including a measure of the GFLV variability baseline, must be done prior to the release of transgenic grapevines. The results showed that the three investigated genes are differently variable, with the largest variability being 13.2 % in the case of 2C. A recombination event was identified in one of the investigated genes. We confirmed the presence of more than one GFLV restrictotype in a single plant. Furthermore, we have selected grapevines infected with GFLV for detailed recording of visual symptoms.
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