Coagulase gene polymorphism of Staphylococcus aureus isolated from subclinical bovine mastitis | Subklinik inek mastitislerinden izole edilen Staphylococcus aureus suşlarının koagulaz gen polimorfizmi
2007
Aslantaş, Ö., Mustafa Kemal Univ., Faculty of Veterinary Medicine, Hatay (Turkey). Div. of Microbiology | Demir, C., Mustafa Kemal Univ., Faculty of Veterinary Medicine, Hatay (Turkey). Div. of Microbiology | Türütoğlu, H., Mehmet Akif Ersoy Univ., Faculty of Veterinary Medicine, Burdur (Turkey). Div. of Microbiology | Cantekin, Z., Mustafa Kemal Univ., Faculty of Veterinary Medicine, Hatay (Turkey). Div. of Microbiology | Ergün, Y., Mustafa Kemal Univ., Faculty of Veterinary Medicine, Hatay (Turkey). Div. of Obstetrics and Gynecology | Doğruer, G., Mustafa Kemal Univ., Faculty of Veterinary Medicine, Hatay (Turkey). Div. of Obstetrics and Gynecology
英语. This study was conducted to investigate the coagulase gene polymorphism of Staphylococcus aureus isolated from subclinical bovine mastitic milk samples from 3 different Turkish cities (Hatay, Gaziantep, and Burdur). Amplification of the coagulase gene from 80 S. aureus isolates produced 5 different polymerase chain reaction (PCR) products; 79 isolates showed only 1 amplicon, and 1 isolate showed 2 amplicons. The isolates were grouped into 9 genotypes by analysing the restriction fragment length polymorphism (RFLP) of the gene; the 2 most common genotypes accounted for 73.8% of the isolates. The results indicated that many coagulase gene genotypes are present in the studied regions and only 1 or 2 genotypes predominated.
显示更多 [+] 显示较少 [-]土耳其. Bu çalışma, üç farklı şehirden (Hatay, Gaziantep ve Burdur) toplanan mastitisli sığır sütlerinden izole edilen 80 Staphylococcus aureus suşunun koagulaz gen polimorfizmini araştırmak amacıyla yapıldı. Seksen S. aureus suşu 5 farklı polymerase chain reaction (PCR) amplikonu oluştururken, 79 izolat 1 amplikon ve 1 izolat 2 amplikon oluşturdu. İzolatlar, koagulaz geninin restriction fragment length polymorphism (RFLP) analizi ile 9 genotipe ayrıldı. İki yaygın genotip izolatların % 73,8'ini oluşturdu. Sonuçlar, çalışılan bölgelerde farklı genotiplerin olduğunu ve bunlardan sadece bir veya iki genotipin dominant olduğunu göstermektedir.
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