Оценка эффективности получения эмбрионов в системе in vitro в условиях разной концентрации СО2 в атмосфере
2010
Golubets, L.V. | Starovojtova, M.P., Grodno State Agrarian Univ. (Belarus)
From the research results it is established that the optimum СО2 concentration in the atmosphere is 5%, which allows one to get up to 22,4% of blastocysts from a number of splitting up germs. The decrease in the carbonic gas concentration with growing and developing cells enhances the yield of blastocysts (embryos) in comparison with the control by 6,9%. The use of the Menezo medium has shown a higher efficiency in comparison with ТС-199 as the СО2 concentration in the surrounding air is decreased. At the 4% СО2 concentration the yield of blastocysts is increased by 20,8%, at the 3% concentration, when the medium ТС-199 is used, morulas did not develop to the next stage.
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