Influence of sterilizing agents and nutrient medium on viability and growth of meristem tips of gooseberry cultivars in vitro culture | Влияние стерилизующих соединений и питательной среды на жизнеспособность и развитие меристиматеческих верхушек сортов крыжовника в культуре in vitro
2009
Kolbanova, E.V., National Academy of Sciences. Scientific and Practical Center for Potato, Vegetable and Fruit Growing (Belarus). Institute for Fruit Growing
Terminal and axillary buds of one-year old woody shoots of cultivars Ravolt Masheka, Kurshu dzintars, Malahite in a phase of a profound rest (since October, 31st - till November, 13th) were used to obtain a shoot culture of gooseberry gooseberry (Ribes uva-crispa) in the conditions of the Republic of Belarus. In the form of sterilizing agents there were tested 0.1% mercuric chloride solution, 10% chloramin solution and 33% hydrogen peroxide solution in combination with treatment of 70% ethanol and 0.5% oxihom solution. Explants were cultivated on five modifications of nutrient medium MS. It was established that MS nutrient medium modification containing full norm of macrosalts were not suitable for gooseberry morphogenesis. Decrease of NH4NO3 and KNO3 content in media used in 2 and 3 times increased regenerative ability of all studied cultivars. The cultivar Masheka had high morphogenetic potential. In this cultivar the quantity of viable explants without taking into account the infected ones in one nutritive media was 97%, in the other – it lowered up to 89,8%, at the same medium the conglomerate shootings were in average 1.16 cm.
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