Effects of soy-isoflavones on ruminal metabolism in vitro | 大豆异黄酮对人工瘤胃代谢的影响
2009
Wang Yongmei, Nanjing Agricultural University, Nanjing(China), Key Laboratory of Animal Physiology and Biochemistiy | Han Zhengkang, Nanjing Agricultural University, Nanjing(China), Key Laboratory of Animal Physiology and Biochemistiy | Wang Guojie, Nanjing Agricultural University, Nanjing(China), Key Laboratory of Animal Physiology and Biochemistiy
中国人. 采用静态人工瘤胃体外培养方法，研究不同质量浓度的2种大豆异黄酮（大豆黄酮和染料木素）对瘤胃微生物代谢的影响。结果表明：当培养液中大豆异黄酮为100mg・L. 1时，大豆黄酮能使瘤胃液中氨氮含量升高2．2mg・L. 1（P〈0．05），微生物蛋白含量提高20．0μg・mL. 1（P〈0．05），总挥发性脂肪酸浓度上升2．7mmol・L. 1（P〈0．05），丙酸／乙酸值为0．74，表明大豆黄酮能促进瘤胃微生物的氮代谢与糖代谢。100mg・L. 1的染料木素使瘤胃液中微生物蛋白含量提高36．8μg・mL. 1（P〈0．01），乙酸比例升高11．4％（P〈0．01），丙酸比例下降7．4％（〈0．01），丁酸比例下降10．2％（P〈0．05），表明染料术素促进了瘤胃微生物的氮代谢，瘤胃发酵类型有向乙酸型转变的趋势。[著者文摘]
显示更多 [+] 显示较少 [-]英语. Mixed ruminal fuid and artifical saliva were incubated in anaerobic media for 12 h in vitro. The soy-isoflavones （daidzein and genistein） were added to the media at different concentrations. The result showed that, when the concentration was 100 mg.L. 1, daidzein could increase the content of NH3-N 2. 2 mg. L. 1 （ P 〈 0. 05 ）, the content of microbial protein （MCP） was enhanced 20. 0 μg.mL. 1 （ P 〈 0. 05 ） , the concentration of total volatile fatty acid （TVFA） was improved 2.7 mmol.L. 1 （ P 〈 0.05 ）. Thisresult demonstrated that daidzein promoted carbohydrates and nitrogen metabolism of ruminal microorganism. At the same concentration, 100 mg.L. 1 genistein could increase the content of MCP 36. 8 μg .mL. 1 （P 〈0. 01 ）, proportion of acetate increased1 11.4% （P〈0. 01）, while propionate decreased 7.4% （P〈0. 01）, and butyrate decreased 10.2% （P〈0.05）. This indicated that genistein promoted nitrogen metabolism, and the ruminal fermentation pattern was become.
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