Distribution of porcine parvovirus in the infected pigs by real-time quantitative PCR | 实时定量PCR探讨猪细小病毒自然感染猪体内病毒的分布情况

中国人. 探讨猪自然感染猪细小病毒(Porcine Parvovirus，PPV)后病毒在体内的分布情况，分析病毒对猪器官组织的嗜性，揭示猪细小病毒的致病机制。利用已建立的猪细小病毒SYBR GreenⅠ实时定量PCR检测方法，定量检测自然感染仔猪、妊娠母猪和同时混合感染有猪圆环病毒2型的仔猪器官组织中PPV DNA的相对含量，分析猪细小病毒的组织嗜性及其与圆环病毒混合感染时的相互作用机制。在多种器官组织中均可检测到PPV，在感染仔猪的肾脏、脾脏、髂下淋巴结中病毒含量较高，为1.39×105～1.81×106 μL－1；在感染妊娠母猪的肾脏、脾脏、肺脏、子宫内膜中病毒含量为4.56×105～5.82×106 μL－1；混合感染PPV和猪圆环病毒2型（PCV2）仔猪肺脏中的PPV DNA相对含量显著增加，从2.69×104 μL－1增加到5.04×105 μL－1。PPV对猪肾脏、脾脏、淋巴结、妊娠母猪子宫的嗜性最强，对肝脏的嗜性较弱；PCV2的混合感染可促进PPV在肺脏的复制增殖，增强了PPV对感染器官组织的亲嗜能力。

英语. In order to survey the distribution of porcine parvovirus in pigs after infection, the research was conducted to investigate the tropism of the virus on pig organs and tissues, and reveal the pathogenic mechanism of PPV. SYBR GreenⅠreal-time quantitative PCR was used to detect the relative content of PPV DNA in infected porcine heart, lung, kidney, spleen, liver, lymphonodi subiliaci, endometrium and other organs. The results showed that the PPV DNA can be detected in heart, liver, spleen, kidney, lung, lymphonodi subiliaci of piglets, especially in kidney, spleen, lymphonodi subiliaci of piglets (1.39×105－1.81×106 μL－1). The relative content of PPV DNA in kidney, spleen, lung, endometrium of pregnancy sows is high up to 4.56×105－5.82×106 μL－1. Comparing the naturally infected piglets with the porcine circovirus type 2 (PCV2) and the PPV mixed infection piglets, the relative content of PPV DNA in lung is increased obviously from 2.69×104 μL－1 to 5.04×105 μL－1. The PPV in the kidney, spleen, lymphonodi subiliaci and endometrium of pregnancy sows has the strongest tropism, but lower for the liver tropism. The existence of PCV2 infection increases the proliferation and replication of the PPV, and enhances the PPV of the pro-infected tissue tropism.