The role of polyamine oxidative degradation in the process of lettuce seed germination | 多胺氧化降解在莴苣种子萌发过程中的作用

[Objective] The aim of this study was to understand the role of polyamine oxidative degradation in the process of lettuce seed germination. [Method] After lettuce seed soaking treatments with aminoguanidine (AG, a specific inhibitor of polyamine oxidases), seed germination rate, activities of polyamine oxidase (PAO) and diamine oxidase (DAO), change of endogenous polyamine and H2O2 content were determined. [Result] Compared with the control, AG treatment strongly inhibited the seed germination, which also had an extremely significant difference in seed germination rate after incubation for 12 h. During the seed germination, activities of PAO and DAO significantly changed, while their activities firstly increased and then decreased, then DAO and PAO reached peaks at 24 h and 48 h respectively. AG treatment was strongly inhibitory for activities of DAO and PAO, whose activities even disappeared after incubation with AG for 24 h and 36 h. During the seed germination, endogenous Put reduced sharply in the first 24 h, then reduced slowly in 24-60 h, while Spd decreased slowly in the early stage of germination, and then sharply declined after 48 h. However, Spm content was low but slightly increased, and the total polyamine gradually decreased. AG treatment could significantly increased endogenous polyamines, especially Put and Spd contents. During the seed germination, H2O2 content gradually increased, and had a peak from 36 to 48 h, then kept a high level at last. AG treatment could significantly reduce H2O2 content. [Conclusion] During the seed germination, the changes of endogenous polyamine and H2O2 content correspond with the changes of PAO and DAO activities, which indicate that there is an active polyamine metabolism of oxidative degradation during the lettuce seed germination.