Optimization of extraction methods of ROP GTPase from young leaves of wheat | 小麦幼叶ROP GTPase提取方法的优化
2010
An Xuliang, Shanxi Normal University, Linfen (China), School of Life Sciences | Han Rong, Shanxi Normal University, Linfen (China), School of Life Sciences
All of the Rho GTPase seems to form a special sub-family, because such a sub-family has so far only found in plants, and then named Rop GTPase, which directly involved in and regulated of muscle actin cytoskeletal reorganization, such as a series of signal transductions. The efficient purification technology and the means of ROP GTPase in wheat are the key basis of the studies on its functions and prosperities. And it has a very important theoretical and practical significance in the signal transduction and F-actin study of ROP GTPase in wheat. Using gel filtration chromatography and non-denaturing polyacrylamide gel electrophoresis with high resolution, ROP protein were extracted, purified and identified in wheat.
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