Study on culturing Prunus salicina cv. Zaoshi embryos | 早食李幼胚离体培养的研究

[Objective] The aim was to study the effective factors on culturing Prunus salicina cv. Zaoshi embryos in vitro. [Method] Different age, culture medium and GA3 which affected culturing of prumssalicina embryos were discussed. [Result] The result showed that on the part of 26-41 d, after the blooming period, the embryos remained tiny and retained endosperms and showed no signs of change after having cultured for three generations. On the part of 48 d, after the blooming period, the endosperms had disappeared, the embryos kept growing until they filled the seed cavity; when they were planted on the MS culture medium, their survival rate reached 77%, in its first generation, the response of embryos was discernible. On the part of 65 d, after the blooming period, 4.5% of their embryos grew into shoots on the MS culture medium; with the age of embryos growing, the survival rate of shoots increased until it reached 26% when the fruits went into ripeness; the embryos produced calli in their first generation of culturing. On the part of 65-83 d, after the blooming period, the embryos produced calli through more than 2-3 generations. On the part of 88 d, after the blooming period, the survival rate of shoots on the WPM culturing base doubled compared with that on the MS culturing base; on the same culture medium, the embryos were inhibited from growing into shoots when BA, KT or 2, 4-D was added on to the culture medium. The survival rate of shoots was increased remarkably when the seeds were treated in 1 000mg/L GA3. [Conclusion] This study provided experimental basis for the establishment of Prunus salicina cv.Zaoshi, embryos rescue techniques and cross breading.