Isolation, screening and identification of cellulose decomposing strains in straw-amended soil | 玉米秸秆还田土壤中纤维素分解菌的分离筛选和鉴定
2010
Guo Yan, Shangqiu Normal University, Shangqiu (China), Department of Life Science | Zhang Jinliang, Shangqiu Normal University, Shangqiu (China), Department of Life Science | Wang Huiping, Shangqiu Normal University, Shangqiu (China), Department of Life Science
[Objective]The aim was to isolate cellulose decomposing strains with high activity from straw-amended soil. [Method]Screening of CMC solid culture and shaking culture rescreening were carried out to obtain cellulose decomposing strains with high activity from the corn straw amended soil, which were analyzed by 16rDNA sequence analysis. [Result] A bacterium and a fungus with higher endonucleases activity were obtained through preliminary screening and secondary screening, a fungus with higher filter paper enzyme activity, and a bacterium (NO.5 strain) with higher of both filter paper enzyme activity and CMC enzyme activity were obtained. The result of 16S rDNA sequence analysis showed that the similarity was 100% between NO.5 strain and Bacillus subtilis. [Conclusion] NO.5 strain was identified as Bacillus subtilis.
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