Change of Storage Substance Contents and Sucrose-metabolizing Enzyme Activities on GA_3 induced during the Variable-temperature | 变温层积过程中GA3诱导天女木兰种子贮藏物质及糖代谢关键酶活性的变化

中国人. 对天女木兰（M.sieboldiiK.Koch）种子进行变温层积处理,测定其在GA3处理诱导下种子内淀粉、糖含量以及糖代谢关键酶的活性。结果表明：在GA3诱导下,淀粉作为一种暂时储存的物质不断地降解,层积结束后CK处理淀粉含量最少,降至0.43mg•g. 1;层积前期（40d）与层积后期（120d）以积累蔗糖为主,以层积40d时CK处理蔗糖含量最大,其值高达2.3mg•g. 1,同期酸性转化酶（AI）和中性转化酶（NI）活性降低,蔗糖合成酶（SS）和蔗糖磷酸化酶（SPS）活性升高;层积中期（90d）果糖和葡糖糖含量随GA3浓度的增加有增强的趋势,以1500mg•L. 1 GA3处理果糖和葡萄糖含量最高,分别为0.33mg•g. 1和0.38mg•g. 1,此时AI和NI活性达到最大值。

英语. In order to understand the characterristics of suger metabolism in M.sieboldiiK.Koch seeds,monitored the changes of starch,suger and sucrose metabolism enzyme activities were monitored on GA3 induced of M.sieboldiiK.Koch seeds during variable-temperature stratification process.The results showed that the starch as a temporary substance was decomposed continuously on GA3 induced during the stratification process,starch content of CK treatment reduced the minimum value afeter stratification process（0.43 mg•g-1）.Sucrose were predominantly accumulated in the early stratification stages（40 days） and late stratification stages（120 days）,suger content of CK treatment reduced the maximum value afeter stratification process（2.3mg•g. 1）.In the same time period the activities of sucrose synthase（SS） and sucrose phosphate synthase（SPS） rised,while the activities of acid invertase（AI） and neutral invertase（NI） reduced.The sucrose content were decomposed continuously;Glucose and fructose content increased with the increasing amount of GA3,and 1500mg•L. 1 of GA3 treatment had the highest content of glucose and fructose,0.33mg•g. 1 and 0.38mg•g. 1,respectivelies.Meanwhile,the activities of AI and NI reached the maximum.