Phenotypes and genetic analysis of a senescence mutanting by aeronautics in rice | 水稻航天衰老突变体基因psl2的表型和遗传分析

中国人. 叶片是植物最主要的光合作用器官,是水稻的源器官。其生长、发育和衰老的分子机理的研究对水稻经济产量和生物产量具有重要的意义。2006年我单位在参加农业部实践8号卫星航天育种工程的空间辐射诱变籼稻（Oryza sativa L.indica）泸恢H103中得到一叶片早衰突变体。初步研究结果表明,该早衰叶突变体表现的特点是：抽穗期前心叶抽出时,先前抽出的倒4叶片就开始变黄衰老,抽穗初期时先前抽出的倒3叶表现衰老,抽穗后期先前抽出的倒2叶表现衰老,灌浆期剑叶表现衰老,完全成熟时剑叶完全衰老死亡。利用该突变体分别与其野生型泸恢H103、R527、R602杂交,获得F1及其衍生的F2群体,对早衰叶突变体进行遗传分析。遗传分析表明由一隐性核基因psl2控制。本研究为最终定位和克隆目标基因奠定了基础。[著者文摘]

英语. Plant leaf is the most important organ for photosynthesis, and it is the source organs of rice. The research on growth, development and molecular mechanism of aging have great significance to the economic and biological yield of rice. We obtained senescence mutant from leaves of the rice Luhui-H103 （Oryza sativa L. indica） after space radiation mutagenesis in the 8th unit of satellite space breeding projects of the ministry of agriculture in 2006. The preliminary findings showed that the representative features of senescence leaves mutant were follow： the four leaves from root which were putting out previously began to turn yellow and aging when the centre leaf put out before heading stage; In the initial stage of heading, the three leaves from root were aging; The two leaves from root were aging at the later stage of heading; Flag leaf was senescent in pustulation period, and the flag leaves were senescent completely and dead when the spikes were full maturity. The mutants were crossed with their wild-type Luhui-H103, R527 and R602, respectively, obtained the F1 and F2 groups, and using them for genetic analysis of premature aging leaf mutants. Genetic analysis showed that the mutant was controlled by a recessive nuclear gene psl2. This research laid the foundation for the final mapping and cloning of the target gene.[著者文摘]