春兰AGL6基因的克隆及实时定量表达分析

Sun Chongbo, Zhejiang Academy of Agricultural Sciences,Hangzhou, Institute of Horticulture; Xiang Lin, Zhejiang Academy of Agricultural Sciences,Hangzhou, Institute of Horticulture; Shi Jisen, Nanjing Forestry University,Nanjing

Cloning and real-time expression analysis of agl6 gene from cymbidium goeringii | 春兰AGL6基因的克隆及实时定量表达分析

2010

Sun Chongbo, Zhejiang Academy of Agricultural Sciences,Hangzhou(China), Institute of Horticulture | Xiang Lin, Zhejiang Academy of Agricultural Sciences,Hangzhou(China), Institute of Horticulture | Shi Jisen, Nanjing Forestry University,Nanjing(China)

中国人. 本研究采用RT-PCR结合RACE技术从春兰（Cymbidium goeringii）中分离到一个AGL6基因。序列分析表明,该基因含有一个729bp长的开放阅读框（ORF）,共编码242个氨基酸。系统进化树分析显示,该基因属于MADS-box基因家族AP1/AGL9组的AGL6同源基因,其编码的蛋白与其它植物AGL6类蛋白具有较高的同源性,命名为CgAGL6（基因登录号为HM208533）。实时荧光定量表达分析表明,CgAGL6基因春兰不同组织中均有表达,其中在唇瓣、花芽和子房中的表达量较高,在花瓣和萼片中的表达量次之,在根、叶和蕊柱中的表达量最低,显示了CgAGL6基因可能在春兰成花转变和花器官的形成过程中起着重要作用。[著者文摘]

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英语. In this research,a novel AGL6 gene was isolated from Cymbidium goeringii by RT-PCR and RACE-PCR techniques.Sequence analysis showed the gene contained an open reading frame of 729 bp encoding a putative protein of 242 amino acids.Homology analysis showed that the deduced protein was high identity with other AGL6 proteins from different species,named CgAGL6（GenBank accession No.HM208533）.Phylogenetic tree analysis also indicated that CgAGL6 belongs to AGL6 clade of AP1/AGL9 subfamily.Real-time quantitative PCR demonstrated that CgAGL6 was expressed in all plant tissues,highly expressed in lips,young buds and ovary,much lower in petals and sepals,and lowest in root,leaves and columns.These results displayed that CgAGL6 may regulate the flowertransition and formation of C.goeringii.[著者文摘]

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