Effects of long-term commercial extenders for liquid storage of boar semen
2010
Lustykova, A.,Vyzkumny Ustav Zivocisne Vyroby, Prague (Czech Republic) | Frydrychova, S.,Vyzkumny Ustav Zivocisne Vyroby, Prague (Czech Republic) | Lipensky, J.,Vyzkumny Ustav Zivocisne Vyroby, Prague (Czech Republic) | Cerovsky, J.,Vyzkumny Ustav Zivocisne Vyroby, Prague (Czech Republic) | Rozkot, M.,Vyzkumny Ustav Zivocisne Vyroby, Prague (Czech Republic)
The objective of this study was to compare the preservation ability of five long-term commercial semen extenders by means of spermatozoa long-term thermo-resistance survival test. Ejaculates from 21 fertile boars were collected by hand method. Semen gel free volume, motility, viability, sperm concentration, total morphologically abnormal spermatozoa and total number of spermatozoa per ejaculate were determined. The samples of diluted sperm in a semen-dilution rate of 1+4 in Androhep (A), Androstar (AS), Androstar plus (AS+), LD and M III were stored at a temperature 17 deg C up to 96 h. The test was performed on 3 mL samples kept at 38 deg C in water bath each day and motility of spermatozoa was evaluated at the 1st, 3rd and 5th hour during the incubation. The survival rate significantly decreased parallelly with the storage length in all the tested extenders. The mean values of sperm motility were 39.66 %, 30.77 %, 28.55 %, 28.67 % and 24.50 %, respectively, in A, AS, AS+, M III and LD. The total mean value of the motility observed with Androhep was significantly higher than those observed with other extenders. In conclusion, the extender Androhep was better than Androstar, M III, Androstar plus and LD extenders in terms of survival rate of boar spermatozoa during long-term liquid preservation.
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