Responses of transgenic Nicotiana tabacum seedlings expressing a Cucurbita pepo antisense PHYA RNA to far-red radiation
2011
Gapeeva, T.A.,National Academy of Sciences of Belarus, Minsk (Belarus). Inst. of Biophysics and Cellular Engineering | Antsipava, T.V.,National Academy of Sciences of Belarus, Minsk (Belarus). Inst. of Biophysics and Cellular Engineering | Pundik, A.N.,National Academy of Sciences of Belarus, Minsk (Belarus). Inst. of Biophysics and Cellular Engineering | Volotovski, I.D.,National Academy of Sciences of Belarus, Minsk (Belarus). Inst. of Biophysics and Cellular Engineering
The Nicotiana tabacum transgenic plants expressing a Cucurbita pepo antisense PHYA RNA were obtained. The seedlings of transgenic tobacco with reduced phytochrome A (PHYA) content displayed decreased sensitivity to continuous broad-band far-red radiation (more than 680 nm). Under far-red irradiance transgenic seedlings showed less elongation of the hypocotyls, more rapid plastid development, more chlorophyll accumulation, less repression of light-dependent NADPH:protochlorophyllide oxidoreductase than wild-type plants. It was in accordance with PHYA control of plant development. Dynamics of the far-red radiation dependent changes in low temperature chlorophyll fluorescence spectra for the transgenic and wild-type seedlings were consistent with the more rapid formation of photosynthetic apparatus in the seedlings with reduced PHYA.
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