Multiplication of echinacea purpurea L. moench by using tissue culture technique
2010
Sakr, S. | Abdou, A. | Kotb, Sh. | Eid, N.
This study was conducted in the Plant Tissue Culture Laboratory, Horticulture Research Institute in April 2009 to determine the best concentration of Thidiazorone (TDZ) hormone to be used in the media on which explants of Echinacea purpurea are cultured during the multiplication stage. Coated and de-coated seeds were sterilized using commercial sodium hypochlorite or hydrogen peroxide, germinated in vitro under darkness or light conditions. The resulting seedlings were used as a source for sterilized explants and used in callus induction. Different kinds of explants (leaves, stems and roots) were cultured on MS media supplemented with different concentrations of TDZ (0,0.1,0.5,1,2, 3,4 and 5 mg/L). After 45 days, the callus resulting from each of the TDZ treatments were either subcultured on of MS hormone -free medium or Ms medium with the same TDZ concentrations used during callus induction. The present results showed that the highest percentage of seed germination was 38.89% obtained when coated seeds were sterilized with H202 and incubated in light. Leaf explants were the most responding to callus initiation medium and the highest fresh weight value resulted from applying 2 mg/L TDZ, followed by stem explants at lmg/L. The highest number of shoots (3 shoots / leaf explant) was obtained from leaf explants when subcultured on the media containing the same concentration of hormone (1 mg/L TDZ).
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