Study of the effect of a cinnamaldehyde and garlic oil combination (Next Enhance 300) on rumen fermentation profile and methane production | Estudio del efecto de una combinación de cinamaldehído y aceite de ajo (Next Enhance 300) sobre el perfil de fermentación ruminal y producción de metano
2011
Blanch, M. | Cardozo, P.W. | Carro, M.D., Universidad de León (España). Dept. de Producción Animal | Ranilla, M.J., Universidad de León (España). Dept. de Producción Animal
The objective of this study was to analyze the effects of 3 doses (200, 300 and 400 mg of product/L) of NEXT Enhance 300 (NE300; containing cinnamaldehyde and garlic oil) on in vitro ruminal fermentation. Batch cultures (120 mL bottles) of mixed ruminal microorganisms (BCRM) were used to test the effects of the additive. Three hundred mg of 60:40 alfalfa hay:concentrate diet was used as a basal substrate. Rumen fluid was obtained from 4 rumencannulated Merino sheep fed the same diet incubated in BCRM, mixed and strained through 4 layers of cheesecloth into an Erlenmeyer flask with an O2-free headspace. Particle-free fluid was mixed with the buffer solution of Goering and Van Soest (1970; no trypticase added) in a proportion 1:4 (vol/vol) at 39 deg C under continuous flushing with CO2. Thirty mL of buffered rumen fluid were added into each bottle under CO2 flushing and were sealed with rubber stoppers and aluminium caps, and incubated at 39 deg C. After 24h of incubation, total gas production was measured, and a gas sample was removed for methane production. Bottles were then uncapped, the pH was measured immediately, and samples for volatile fatty acid (VFA) and ammonia-N analyses were taken. Incubations were repeated on 4 different days. Differences were declared at P < 0.05. Dose of 400 mg/L of NE300 decreased total VFA production and apparently fermented organic matter compared to control (CTR, no additive), thus indicating some inhibition of ruminal fermentation. NE300 at 200 mg/L reduced acetate:propionate ratio, methane production and methane/VFA ratio compared to CTR. NE300 at 300 mg/L reduced ammonia-N concentrations (P = 0.069), methane production, acetate proportion, and acetate:propionate ratio, and increased propionate proportion compared to CTR. In conclusion, NE300 at 300 mg/L decreased methane production and increased propionate proportion without affecting total VFA production, and this would indicate a higher supply of energy for the host animal.
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