Detection of Bacterial agent ORT and E.Coli in Suspected poultry flocks in southern khorasan province.

The characteristic features of an O. rhinotracheale infection include relatively mild respiratory signs in young birds. These symptoms start with sneezing and are accompanied by a slightly increased mortality and a poor performance. Asymptomatic infection or without obvious clinical signs and post-mortem lesions of the different O. rhinotracheale infections are not sufficiently specific to be diagnostic or are minimal or absent. The organism is a gram-negative, pleomorphic, non-motile rodshaped bacterium which grows aerobically, microaerobically, or anaerobically, meanwhile Due to the small size of the colonies upon culture and frequent contamination during sample collection masking by other bacterial agents typically distorts identification and may result in faulty diagnosis. Escherichia coli is a part of the common microbial flora of the intestine of poultry and most isolates are nonpathogenic. About 10 to 15% of intestinal coliforms are pathogenic serotypes. Pathogenic E. coli are also present in the poultry environment. Escherichia coli causes a variety of lesions in poultry, including yolk sac infection, omphalitis, cellulitis, swollen head syndrome, coligranuloma, and colibacillosis . In this study a total of 13 lung and trachea samples were collected from 180000 clinically infected broiler flocks at ages of 1-42 day showing respiratory disease symptoms. In the present work PCR and culture was used for identification ORT and Isolation, Identification and serotyping for E.Coli in the samples of broiler breeder flocks. Furthermore, at the end of the investigation, it was determined that samples taken from flocks in the present study were negative by PCR test and culture. Serogrouping of the E. coli isolates was done at the Razi Vaccine and Serum Research Institute and serotyping of 13 samples Representing 13 broiler flocks by using Mast Diagnostic kit UK, was done for E.Coli which ten isolates were under serogroups O2 whereas 3 isolates could not be typed. The main objective was to evaluate the infectious of ORT and E.Coli in chickens at 1-42 ages, in other hand we demonstrate for the first time that the prevalence of O. rhinotracheale is low, meanwhile majority infectious of E.Coli were under serogroups O2 in broiler breeder flocks in southern khorasan province. The purpose of this study was to examine ORT from chickens by culture and PCR tests, in addition identification and serotyping of E.Coli Using specific antiserum which was also done too, in southern khorasan province.