In vitro investigation of Aspergillus flavus inhibition growth and Aflatoxin B1 uptake by Lactobacillus plantarum
2009
Khanafari, A., Microbiology Department, Islamic Azad University North of Tehran branch-Iran | Soudi, H,, Plant disease Research Department, Plant and Diseases Research Institute- Tehran-Iran | Miraboulfathi, M,, Plant disease Research Department, Plant and Diseases Research Institute- Tehran-Iran | Karami Osboo, R., Plant disease Research Department, Plant and Diseases Research Institute- Tehran-Iran
Laboratory studies assessed the binding of aflatoxin B1 (AFB1) and its adsorption by many strains of lactic acid bacteria which werw used in foods. In this study the binding ability of AFB1 ? a main endoded Lactobacillus plantarum (PTCC 1058) was studied. The 0.5 ppm AFB1 solution was inoculated at 37ºC for 1, 24, 48, 90 hours and the AFB1 residues were quantitively in supernatant by HPLC. Results showed this strain removed 45% of AFB1 from solution within 1h and 100% of it in 90h. In death phase, this bacteria removed only 31% of AFB1 from solutions after 1 and 24h, but this value reduced to 15% after 48 and 90h incubation periods (P0.05). The stabilities of the amount of AFB1 to bacterium cell wall were evaluated by usign organic solvent (chloroform) and the result indicates no AFB1 extraction by solvent. The low amount aflatoxin production by three different methods represented high level of fungus growth suppression by lactobacillus.
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