Antioxidant Activity of Methanol Extracts from Cudrania tricuspidata Bureau according to Harvesting Parts and Time
2009
Choi, S.R., Medicinal Plants Research Institute, Jeollabukdo ARES, Jinan, Republic of Korea | You, D.H., Medicinal Plants Research Institute, Jeollabukdo ARES, Jinan, Republic of Korea | Kim, J.Y., Medicinal Plants Research Institute, Jeollabukdo ARES, Jinan, Republic of Korea | Park, C.B., Medicinal Plants Research Institute, Jeollabukdo ARES, Jinan, Republic of Korea | Kim, D.H., Medicinal Plants Research Institute, Jeollabukdo ARES, Jinan, Republic of Korea | Ryu, J., Jeollabukdo Agricultural Research and Extension Services, Iksan, Republic of Korea
To obtain the information on antioxidant activity by harvesting parts and time in Cudrania tricuspidata Bureau, total phenolic contents, flavonoid contents, superoxide dismutase (SOD) like activity and 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical scavenging activity of methanol extracts from Cudrania tricuspidata were investigated. Total phenolic contents was the highest in leaf [30.2~38.8 mg/g dry weight (DW)] and followed by root bark, stem bark, fruit, root except bark and stem except bark. Among harvesting time, total phenolic contents of leaf was the highest as 38.1~38.8 mg/g DW in May and June, and then decreased to October. Root bark harvested in April and May contained 26.6~27.8 mg/g DW total phenolic compounds and total phenolic content fell to a very low values at 1.8 mg/g DW in February. Flavonoid contents was very high in leaf and root bark. Especially, root bark harvested in April had the highest of flavonoid contents as 23.2 mg/g DW. As a result of simple linear regression analysis of total phenolic contents on flavonoid contents according to harvesting parts, it showed a high correlation (p less than 0.001) with 0.57~0.97 R² (coefficient of determination). As RC∧50 value, that was, the concentration of sample required for 50% reduction of DPPH absorbance, was 13.7~20.5 ㎍/mL in leaf so DPPH radical scavenging activity was very high. But SOD like activity of 1,000 ㎍ extract/mL MeOH was a low value of 0.3~9.2% in all samples.
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