Microbial Community Changes in the Soil of Plastic Film House as Affected by Anaerobic Fermentation of Rice Bran or Wheat Bran
2009
Kim, H.L., Namhae Sub-Station, National Institute of Horticultural and Herbal Science, RDA, Namhae, Republic of Korea | Weon, H.Y., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Sohn, B.K., Sunchon National University, Sunchon, Republic of Korea | Choi, Y.H., Namhae Sub-Station, National Institute of Horticultural and Herbal Science, RDA, Namhae, Republic of Korea | Kwack, Y.B., Namhae Sub-Station, National Institute of Horticultural and Herbal Science, RDA, Namhae, Republic of Korea
Soil microbial community has been changed after the treatment of anaerobic fermentation using wheat bran or rice bran was applied to the soil. In the dilution plate technique, the number of anaerobic bacteria and fungi was higher in rice bran-treated soil than in non and wheat bran-treated soil, but of yeast was higher in wheat bran-treated soil than in non and rice bran-treated soil. Specially, the fungi were not detected in the wheat bran-treated soil. Identified by 16S rDNA sequencing, the number of aerobic bacteria was similar in all treatments, the dominant bacteria was the genus Bacillus. In the phospholipid fatty acid (PLFA) technique, both Gram-positive and Gram-negative bacteria change slightly in all treatments for 20days of fermentation process but, after 20day, increased rapidly in wheat or rice bran-treated soil. In conclusion, the microbial communities structure was dramatically changed after the treatment of wheat or rice bran to soil.
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