Quantitative Analyses for the Quality Evaluation of Salviae Miltiorrhizae Radix by HPLC
2010
Fang, Zhe, Catholic University of Daegu, Gyungsan, Republic of Korea | Moon, D.C., Chungbuk National University, Cheongju, Republic of Korea | Son, K.H., Andong National University, Andong, Republic of Korea | Son, J.K., Yeungnam University, Gyeongsan, Republic of Korea | Min, B.S., Catholic University of Daegu, Gyungsan, Republic of Korea | Woo, M.H., Catholic University of Daegu, Gyungsan, Republic of Korea
In this study, quantitative analysis for the quality evaluation of Salviae Miltiorrhizae Radix using HPLC/UV was developed. For quantitative analysis, six major bioactive compounds were determined. The separation conditions employed for HPLC/UV were optimized using ODS C∧18 column (250 × 4.6 mm, 5 ㎛) with gradient condition of A (1% formic acid in H₂O) and B (acetonitrile : methanol : formic acid = 100 : 75 : 1) as the mobile phase at a flow rate of 1.0 mL/min and a detection wavelength of 280 nm. These methods were fully validated with respect to the linearity, accuracy, precision and recovery. The HPLC/UV method was applied successfully to the quantification of six major compounds in the Salviae Miltiorrhizae Radix. The results indicate that the established HPLC/UV method is suitable for the quantitative analysis.
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