Evaluation of NPTII Gene Transfer from Transgenic Chili Pepper to Culturable Microorganisms in Rhizosphere Soil
2010
Lee, B.K., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Park, J.Y., Bio-Evaluation Center, KRIBB, Cheongwon, Republic of Korea | Park, K.W., Bio-Evaluation Center, KRIBB, Cheongwon, Republic of Korea | Harn, C.H., Nongwoo Bio Co., Ltd., Yeoju, Republic of Korea | Kim, H.M., Bio-Evaluation Center, KRIBB, Cheongwon, Republic of Korea | Kim, C.G., Bio-Evaluation Center, KRIBB, Cheongwon, Republic of Korea
The possible gene transfer from transgenic chili pepper to soil bacteria in rhizosphere was evaluated. DNA was extracted from rhizosphere soil collected from growing transgenic chili pepper (Capsicum annuum L.) resistant to cucumber mosaic virus (CMV) and control line, and PCR analysis was performed with specific primer for transgene in the transgenic chili pepper. The results of the PCR targeting NPTII gene indicated that the transgene was present in the rhizosphere soil samples in all collecting times. To investigate the cause of the transgene detection in the samples, we isolated microorganisms from the soil and cultured them on bacterial and fungal medium containing kanamycin. About 430 thousands of bacterial colonies and 160 thousands of fungal colonies were investigated, however, colonies resistant to kanamycin were not detected, indicating gene transfer from transgenic chili pepper to culturable rhizosphere soil microorganisms was not occurred in the present study.
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