Proteomic Analysis of Differentially Expressed Plasma Proteins during Early Pregnancy in Hanwoo
2010
Kim, P.H., National Institute of Animal Science, RDA, Suwon, Republic of Korea | Kwon, D.J., National Institute of Animal Science, RDA, Suwon, Republic of Korea | Oh, K.B., National Institute of Animal Science, RDA, Suwon, Republic of Korea | Lee, H.C., National Institute of Animal Science, RDA, Suwon, Republic of Korea | Yang, B.C., National Institute of Animal Science, RDA, Suwon, Republic of Korea | Im, G.S., National Institute of Animal Science, RDA, Suwon, Republic of Korea | Min, K.S., Hankyong National University, Ansung, Republic of Korea | Yoon, J.T., Hankyong National University, Ansung, Republic of Korea | Jin, D.I., Chungnam National University, Daejeon, Republic of Korea | Park, S.B., National Institute of Animal Science, RDA, Suwon, Republic of Korea | Hwang, S.S., National Institute of Animal Science, RDA, Suwon, Republic of Korea
This study was performed to comprehend the plasma proteins expressed specifically during early pregnancy in pregnant or non-pregnant Hanwoo using proteomic analysis technique. Plasma samples (0, 2, 3, 4, 7, and 11 weeks after AI) were obtained from pregnant (P, n=3) or non-pregnant (NP, n=4) Hanwoo, respectively. To evaluate proteins differentially expressed, 2-dimensional electrophoresis (2DE) was conducted. Normalized protein spots were selected for the significant expression variation deviated over two fold in its expression level between two groups. Molecular functions of the proteins were DNA binding, protein binding, hemoglobin binding, ferrochelatase and transporter activity and arylestera, respectively. According to western blotting, haptoglobin was specifically expressed only in NP group during early pregnancy; however, paraoxonase 1 was highly expressed in pregnant group. Based on these results, pregnancy was maintained successfully by the activation of specific plasma proteins associated with immune system and antioxidant regulation during early pregnancy in Hanwoo.
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