Studies on Characteristics and Freezing Tolerance of Spermatozoa in Jindo Dog
2010
Choi, S.H., National Institute of Animal Science, RDA, Namwon, Republic of Korea | Kim, S.J., National Institute of Animal Science, RDA, Namwon, Republic of Korea | Cho, S.R., National Institute of Animal Science, RDA, Namwon, Republic of Korea | Choe, C.Y., National Institute of Animal Science, RDA, Namwon, Republic of Korea | Son, J.K., National Institute of Animal Science, RDA, Namwon, Republic of Korea | Kim, J.S., Korean Jindo Dog Center, Jindo, Republic of Korea | Oh, S.I., Korean Jindo Dog Center, Jindo, Republic of Korea | Park, B.J., Korean Jindo Dog Center, Jindo, Republic of Korea | Kim, S.H., Korean Jindo Dog Center, Jindo, Republic of Korea
This study was performed to investigate the characteristics within ages and freezing tolerance of spermatozoa in Jindo Dog. Experimental animals were selected 12 herds within 1~8 year's old and collected semen for 2 times in a week. Collected semen was evaluated whole volume and sperm number with CASA system (SIAS, Medical Supply, Korea). Then seminal plasma were separated and diluted with modified Tris-egg yolk extender and added 4, 6 and 8% glycerol for 4 times to final concentration and equilibrated for 1.5 hrs. Before and after freezing, equilibrated semen were evaluated the survival rates. Total volume of sperm at 1~2 year old group is as 5.2×10∨8 cells/ml largest and there were no significance among groups. The motility of 1~2 year old group is highest as 90.9% and there were significance among groups. Abnormal sperm showed similar among groups. The survival rate in terms of pre-freezing and post-freezing were decreased all levels of glycerol and reveled 87.0% to 64.5% in 4%, 87.5% to 51.9% in 6% and 73.4% to 29.7% in 8%, there were significant difference among the groups (p less than 0.05). These results suggest that the optimal sperm-freezing methods in Jindo Dog are utilized with modified Tris egg-yolk extender with 4% glycerol and were improve the reproductive activity by these methods.
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