Rheinheimera aquatica sp. nov., Antimicrobial Activity-Producing Bacterium Isolated from Freshwater Culture Pond
2010
Chen, Wen-Ming, National Kaohsiung Marine University, Kaohsiung, Taiwan | Lin, Chang-Yi, National Kaohsiung Marine University, Kaohsiung, Taiwan | Young, Chiu-Chung, National Chung Hsing University, Taichung, Taiwan | Sheu, Shih-Yi, National Kaohsiung Marine University, Kaohsiung, Taiwan
A bacterial strain designated GR5∨T, previously isolated from a freshwater culture pond in Taiwan while screening for bacteria for antimicrobial compounds, was characterized using a polyphasic taxonomic approach. Strain GR5∨T was found to be Gram-negative, aerobic, greenish-yellow colored, rod-shaped, and motile by means of a single polar flagellum. Growth occurred at 10-40℃ (optimum, 35℃), pH 7.0-8.0 (optimum pH 8.0), and with 0-2.0% NaCl (optimum, 0.5-1.0%). The major fatty acids were C∧16:1ω7c (36.3%), C∧16:0 (16.6%), C∧12:0 3-OH (12.5%), and C∧18:1ω7c (9.1%). The major respiratory quinone was Q-8, and the DNA G+C content of the genomic DNA was 51.9 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain GR5∨T belongs to the genus Rheinheimera, where its most closely related neighbors are Rheinheimera texasensis A62-14B∨T and Rheinheimera tangshanensis JA3-B52∨T with sequence similarities of 98.1% and 97.5%, respectively, and the sequence similarities to any other recognized species within Gammaproteobacteria are less than 96.5%. The mean level of DNA-DNA relatedness between strain GR5∨T and R. texasensis A62-14B∨T, the strain most closely related to the isolate, was 26.5±7.6%. Therefore, based, on the phylogenetic and phenotypic data, strain GR5∨T should be classified as a novel species, for which the name Rheinheimera aquatica sp. nov. is proposed. The type strain is GR5∨T (=BCRC 80081∨T= LMG 25379∨T).
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