Distribution of DArT Markers in a Genetic Linkage Map of Tomato
2010
Truong, Hai Thi Hong, AVRDC-The World Vegetable Center, Taiwan, Republic of China | Graham, Elaine, AVRDC-The World Vegetable Center, Taiwan, Republic of China | Esch, Elisabeth, Institute for Plant Genetics, Molecular Plant Breeding, Leibniz University of Hannover, Hannover, Germany | Wang, Jaw-Fen, AVRDC-The World Vegetable Center, Taiwan, Republic of China | Hanson, Peter, AVRDC-The World Vegetable Center, Taiwan, Republic of China
A genetic linkage map was constructed using 188 F∧9 RILs derived from a cross between Solanum lycopersicum H7996 (resistant to bacterial wilt) and S. pimpinellifolium WVa700 (highly susceptible to bacterial wilt). The map consisted of 361 markers including 260 DArTs, 74 AFLPs, 4 RFLPs, 1 SNP, and 22 SSRs. The resulting linkage map was comprised of 13 linkage groups covering 2042.7 cM. The genetic linkage map had an average map distance between markers of 5.7 cM, with an average DArT marker density of 1/7.9 cM. Based on the distribution of anchor SSR markers, 11 linkage groups were assigned to 10 chromosomes of tomato except chromosomes 5 and 12. The DArT markers were distributed across the genome in a similar way as other markers and showed the highest frequency of clustering (38.8%) at less-than or equal to 0.5 cM intervals between adjacent markers, which is 3 times higher than AFLPs (13.5%). The present study is the first utilization of DArT markers in tomato linkage map construction.
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