Discrimination of Korean Apple Cultivars Using Combination of RAPD-SCAR Markers
2010
Cho, K.H., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea | Heo, S., Apple Experiment Station, National Institute of Horticulture and Herbal Science, RDA, Gunwi, Republic of Korea | Ki, H.R., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea | Kim, J.H., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea | Shin, I.S., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea | Han, S.E., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea | Kim, S.H., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea | Kim, D.H., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea
Conventional methods for identification of apple cultivars are based on the evaluation of sets of morphological characteristics, however, closely related cultivars often cannot be distinguished by morphological traits. This study was conducted to develop DNA markers for discrimination of the apple cultivars bred in Korea. Thirty random primers generated eighty-three random amplified polymorphic DNA (RAPD) markers from thirty-one Korean bred and introduced apple cultivars. Fifty-two RAPD fragments were cloned and sequenced for conversion into sequence characterized amplified region (SCAR) markers. Among them only seventeen SCAR markers resulted in the amplification of single major bands the same size as the RAPD fragment cloned. Several combinations of six (AN11_433, AN08_566, A408_592, AK17_653, AO04_711, AO04_779 or AW15_368, AN11_433, A408_592, AK17_653, AO04_711, AO04_779, or AL1_427, AN11_433, AN08_566, A408_592, AK17_653, AO04_779) to seven (AL1_427, AN11_433, AN08_566, A408_592, AK17_653, AM16_708, AO04_779 or A330_424, AN11_433, AG14_502, AN08_566, A408_592, AK17_653, AO04_779 or A330_424, AN11_433, AK14_564, A408_592, AK17_653, AM16_708, AT14_789) SCAR markers provided enough polymorphism to identify sixteen Korean apple cultivars among thirty-one tested cultivars. Therefore, application of the seventeen SCAR markers was sufficient to identify the thirty-one tested apple cultivars. These markers could be utilized as a reliable tool for cultivar discrimination of Korean apples.
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