Molecular detection of Aeromonas hydrophila isolated from albino catfish, Clarias sp. reared in an indoor commercial aquarium
2010
Choresca Jr. Casiano H., Seoul National University, Seoul, Republic of Korea | Gomez, Dennis K., University of the Philippines in the Visayas, Miagao, Iloilo, Republic of the Philippines | Han, J.E., Seoul National University, Seoul, Republic of Korea | Shin, S.P., Seoul National University, Seoul, Republic of Korea | Kim, J.H., Seoul National University, Seoul, Republic of Korea | Jun, J.W., Seoul National University, Seoul, Republic of Korea | Park, S.C., Seoul National University, Seoul, Republic of Korea
Moribund albino catfish, Clarias sp., displayed from an indoor private commercial aquarium were submitted in the laboratory for diagnostic examination. Dense culture of bacteria was recovered from the kidney and was characterized using Vitek System 2 and showed 98% probability to Aeromonas (A.) hydrophila. PCR result showed positive using A. hydrophila extracellular hemolysin gene ahh1 (130 bp) and aerolysin gene aerA (309 bp). The 16S rRNA gene was identical and exhibited 97% sequence similarity with the other known isolates of A. hydrophila available in the GenBank. In this paper, we reported the isolation and molecular detection of A. hydrophila from an albino catfish.
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