Molecular and biochemical assessment of Brassica napus and indigenous Campestris species
2008
Abbas, S.J. (NWFP Agricultural Univ., Peshawar (Pakistan). Inst. of Biotechnology and Genetic Engineering) | Farhatullah (NWFP Agricultural Univ., Peshawar (Pakistan). Dept. of Plant Breeding and Genetics) | Khan, I.A. (NWFP Agricultural Univ., Peshawar (Pakistan). Inst. of Biotechnology and Genetic Engineering) | Marwat, K.B. (NWFP Agricultural Univ., Peshawar (Pakistan). Dept. of Weed Science) | Munir, I.
In Pakistan, Brassica is the second most important source of oil after cotton. It contributes about 17% to the domestic production of edible oil. Parental lines along with five F2s were assessed for biochemical parameters using Near Infrared Reflectance Spectroscopy (NIRS). Parental lines contain more oil 45.85% as compared to F2s 42.26% while the F2s contain more protein 25.92% as compared to the parents 23.70%. Both parents and F2 contain high glucosinolate and fatty acids contents. Insulin Growth like Factor (IGF) primer sets were used to estimate genetic relationship among 5 F2 segregating population of Brassica along with 9 parental lines. On an average 29 alleles were amplified using IGF primer sets. Mean genetic distance estimates ranged from 0.25-1.00 (G.D = 0%-100 %), respectively. Size of scorable fragments ranged from approximately 250 to greater than 2000 bp. A high level of genetic dissimilarity (GD= up to 100%) was estimated among all genotypes. Entries were gr
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