Phenolic content in vitro cultures of chickpea (Cicer arietinum L.) during callogenesis and organogenesis
2008
Naz, S. (Lahore Coll. for Women Univ., Lahore (Pakistan). Dept. of Botany) | Ali, A. (University of Sargodha (Pakistan). Dept. of Biological Sciences) | Iqbal, J. (Punjab Univ., Lahore (Pakistan). School of Biological Sciences)
The callogeneic response of different explants i.e. cotyledon, leaf, node, shoot and root apices of Cicer arietinum was investigated on MS basal media supplemented with different concentrations of auxins and cytokinins. A combination of BAP and 2,4-D or NAA enhanced proliferation response. Of different combinations MS+1.0 2,4-D + 0.5 NAA + 0.5 BAP mg/l proved exceptionally good both for callus induction and proliferation. Callus masses initiated from different explants were maintained from main to 10thsub cultures. The morphogenic behaviour of different calli was also investigated. It was observed that the rate of browning and necrosis was increased with the increasing subcultures. To elucidate the biochemical basis of growth inhibition in non-morphogenic and morphogenic calli, total phenolics were estimated from main to 10th sub cultures both quantitatively and qualitatively. A gradual increase in total phenolic content was noticed from main to 10th sub cultures in both non
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