Effect of osmotic stress on proline accumulation, photosynthetic abilities and growth of sugarcane plantlets (Saccharum officinarum L.)
2008
Cha-Um, S. (National Science and Technology Development Agency, Pathumthani (Thailand)) | Kirdmanee, C. (National Science and Technology Development Agency, Pathumthani (Thailand))
Disease-free sugarcane plantlets derived from meristem cutting were photoautotrophically grown on the MS medium and subsequently exposed to 0, 100, 200, 300 or 400 mM mannitol for 7 days. Osmotic pressure in the culture medium was increased with increase in mannitol concentration, causing low water use efficiency (WUE) (r2 = 0.88) and chlorophyll degradation (r2 = 0.92). Chlorophyll a (Chla), chlorophyll b (Chlb) and total carotenoids (Cx+c), concentrations in the osmotic stressed leaves decreased, especially in 400 mM mannitol treatment, degrading 44, 81 and 72%, respectively when compared to control. In contrast, proline content in osmotic stressed plantlets was accumulated and peaked at 2,236.75 mumol g-1 FW in 300 mM mannitol treatment. The WUE and chlorophyll degradation were correlated with maximum quantum yield of PSII (Fv/Fm) (r2 = 0.75) and photon yield of PSII (phi psn) (r2 = 0.83), respectively. The Fv/Fm and phi phi in drought acclimatized plantlets decreased, wh
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