Production of goat pox virus vaccine from a live attenuated goat pox virus strain
2010
Abbas, F. (University of Balochistan, Quetta (Pakistan). Center for Advanced Studies in Vaccinology and Biotechnology) | Khan, F.A. (University of Balochistan, Quetta (Pakistan). Center for Advanced Studies in Vaccinology and Biotechnology) | Hussain, A. (Veterinary Research Inst., Quetta (Pakistan)) | Ahmed, F. | Ahmed, M. | Ahmed, S. | Ahmed, M.Z. | Attique, M.A. | Wadood, A. | Taj, M.K.
Kid kidney cells form less than 2 months of age were grown, 80% confluent monolayers were obtained after 10-12 days. Live attenuated goat pox virus vaccine (Gorgan 55, Iranian) was used to infect the confluent monolayers. Eighty percent cytopethic effect (CPE) was obtained on day 5 of virus inoculation. TCID50 calculated for the pool virus was 10 5.5/ml. Vaccine was found free from any bacterial and mycoplasma contamination. All mice and guinea pigs were found healthy after 21days post vaccinal administration. Vaccinated goats challenged after 14 days with 1 ml of virulent goat pox virus (TCID50 10(4.5)/ml) did not show any unwanted reaction while controls challenged with 1 ml of virulent goat pox virus (TCID50 10(3.5)/ml) showed pox lesions and rise in body temperature. The main objective of this study was to produce and study the efficacy of this vaccine.
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