Production and characterization of keratinase enzyme by culturing Bacillus strain on chicken feathers
2008
Ghafoor, M. (Government Coll. Univ., Lahore (Pakistan). Botany Dept.) | Zaheer-ud-Din, K. (Government Coll. Univ., Lahore (Pakistan). Botany Dept.) | Baig, S. (Pakistan Council of Scientific and Industrial Research Labs., Lahore (Pakistan)) | Saleem, M. (Government Coll. Univ., Lahore (Pakistan). Botany Dept.)
The purpose of the present research was to study the production and characterization of thermostable keratinase from a newly isolated Bacillus strain FBN-4.The study involved two steps. The first step was the production of keratinase enzyme from chicken feathers and the second was to check the stability of the enzyme. The strain of Bacillus sp. FBN-4 was shown to be useful for biotechnological purposes such as for the degradation of chicken feathers. The effect of temperature, pH, incubation time period, different supplementary nitrogen and carbon sources and different concentrations of yeast extract on keratinase production by Bacillus strain FBN-4, were optimized. The enzyme was produced between 33 degree C and 47 degrees C, with maximum activity and yield was achieved at 37 degree C. Enzyme characterization was also demonstrated. This enzyme was almost stable at 60 degree C even after 90 min. of incubation at pH 10.
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