Microsatellite DNA variation in the Siberian sturgeon, Acipenser baeri (Actinopterygii, Acipenseriformes, Acipenseridae), cultured in a Polish fish farm
2010
Fopp-Bayat, D.,University of Warmia and Mazury, Olsztyn (Poland). Faculty of Environmental Science and Fisheries
The application of microsatellite DNA analysis for estimation of gene diversity in the Siberian sturgeon, Acipenser baeri, is described. Fin clips were randomly sampled from 94 specimens of Siberian sturgeon broodstock reared at the fish farm near Konin, Poland. The analysed broodstock has been kept there since 1996, with new specimens being introduced annually. The fish were studied in 2007-2008. Genomic DNA for amplification of microsatellite loci was extracted using Chelex 100. Six microsatellite loci (Afu-19, Afu-39,Afu-68,AfuB-68, Spl-163, andSpl-168) were amplified for examination of the genetic variability of the studied fish. Within 94 individuals of the Siberian sturgeon, a total of 74 alleles were detected in six polymorphic microsatellite loci. The number of alleles per locus ranged from 8 to 18, with an average allele number being 12. The genetic diversity of six microsatellite loci varied from 0.686 to 0.811. This technology has great potential for use in aquaculture of sturgeon fish
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