Genetic analysis of nitrogen accumulation and protein composition in wheat kernel
2008
Charmet, G., Institut National de la Recherche Agronomique-Université B Pascal, Clermont-Ferrand (France). Unité Mixte de Recherche, Génétique, Diversité et Ecophysiologie des Céréales | Martre, P., Institut National de la Recherche Agronomique, Clermont-Ferrand (France). Unité de Recherche Agronomie
The maximum rate and duration for grain dry matter (DM) and nitrogen (N) accumulation were evaluated for 194 recombinant inbred lines (RILs) from a cross between two French wheat cultivars. Grain protein composition was analysed by capillary electrophoresis (CE) and size-exclusion high performance liquid chromatography (SE-HPLC). Correlation analyses revealed that DM and N accumulation rates were closely related, and that protein composition was primarily influenced by the total quantity of N per grain. A genetic map of 254 molecular markers allowed us to identify 7 quantitative trait loci (QTL). Five QTLs were significantly associated with the kinetics of DM and N accumulation, and two of them also influenced protein composition. Two QTLs affected only the protein composition. We also used the SiriusQuality1 wheat simulation model to analyze genotype x environment interactions for grain protein composition. QTLs for the genotypic key parameters of total grain nitrogen partitioning were detected
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