Genetic characterisation and DNA fingerprinting profile of new poplar clones
2010
Galović, V., Institute of Lowland Forestry and Environment, Novi Sad (Serbia) | Orlović, S., Institute of Lowland Forestry and Environment, Novi Sad (Serbia) | Zorić, M., Faculty of Technology, Novi Sad (Serbia) | Zgonjanin-Bosić, D., Clinical Center of Vojvodina, Novi Sad (Serbia). Center for Forensic Medicine, Toxicology and Molecular Genetics | Salatić, I., Clinical Center of Vojvodina, Novi Sad (Serbia). Center for Forensic Medicine, Toxicology and Molecular Genetics | Petrić, G., Clinical Center of Vojvodina, Novi Sad (Serbia). Center for Forensic Medicine, Toxicology and Molecular Genetics
The objective of theis paper was to determined DNA fingerprinting profile for agronomically perspective new ILFE (Institute of Low Forestry and Environment from Novi Sad, Serbia) selected poplar clones using SSR marker system. Genetic profile of 4 poplar clones belonging to Populus deltoides species (Aigeiros section) were evaluated. Sixteen locuses were considered for genetic evalyation and 5 of them revealed considerably high power of discrimination valued 0.75. Results obtained using SSR data showed in total 62 different alleles and 3.87 number of alleles per loci. Cluster analysis shows the closest connection between 182/81 and B229 clones (0.579 similarity). The PE 19/66 clone is genetically more similar to the above clones (0.419) and very distant from B81 clone (0.182). When evaluating polymorphisms within genotypes of interest microsatelite DNA markers proved to be a useful tool for distinguishing genetic background of the clones of interests thus grouping them according to their genetic similarity and consequently successfully reveal their DNA fingerprinting profiles. The results presented in this paper were introduce in ILFE poplar breeding programs, and also could be used as a valuable data in new breeding processes as well as in breeders right protection process.
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