Molecular cloning and characterization of glutamate dehydrogenase cDNA from the giant-embryo Oryza sativa
2010
Xu, H., Shanghai Normal University, Shangai (China). College of Life and Environment Science | Liao, P., Shanghai Normal University, Shangai (China). College of Life and Environment Science | Xiao, J., Shanghai Normal University, Shangai (China). College of Life and Environment Science | Zhang, Q., Shanghai Normal University, Shangai (China). College of Life and Environment Science | Dong, Y., Shanghai Normal University, Shangai (China). College of Life and Environment Science | Kai, G., Shanghai Normal University, Shangai (China). College of Life and Environment Science
Here we report on the cloning and characterization of the OsGAD3 gene from the GABA-rich giant-embryo of rice (Shangshi jing 315, SSJ315). The molecular cloning and characterization of OsGAD3 (SSJ315) showed that it was very similar to other glutamate decarboxylases, indicating that OsGAD3 (SSJ315) belongs to a glutamate decarboxylase superfamily. OsGAD3 (SSJ315) also possesses the typical CaMBD in the C-terminal region like most other plant GADs. Evolution analysis revealed that OsGAD3 (SSJ315) belongs to the plant group and plant GADs are conserved in the process of evolution. This work should be useful to further understand the important step involved in GABA biosynthesis in GABA-rich giat-embryo rice at the molecular level in the near future.
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