Antibacterial effect and cytotoxic effect of supercritical extraction of thyme and thyme extract obtained through hydrodistillation process | Antibakterijsko delovanje i citotoksični efekat natkritičnog ekstrakta timijana i ekstrakta timijana dobijenog procesom hidrodestilacije
2009
Mišić, D., Fakultet veterinarske medicine, Beograd (Serbia) | Nišavić, A., Fakultet veterinarske medicine, Beograd (Serbia) | Žižović, I., Tehnološko-metalurški fakultet, Beograd (Serbia) | Ivanović, J., Tehnološko-metalurški fakultet, Beograd (Serbia)
Investigations of antibacterial effect of supercritical extraction of thyme (TNKE) and thyme extract obtained by hydrodistillation (THD) covered 18 bacteria strains from the genera Staphylococcus, Streptococcus and Enterococcus, including the strains MRSA, the strains VRE, as well as the reference strains S. pyogenes ATCC 19615, S. agalactiae ATCC 27959, S. aureus ATCC 11632, and S. aureus ATCC 25923. The antibacterial effect of thyme extracts was examined using the microdillution method in bouillon, and the cytotoxic effect of thyme extract was examined on the VERO cells line. The obtained values for MIC THD extract ranged from 160 microgram/ml for all bacteria strains covered by the investigations. The obtained values for MIC TNKE extract were 1280 microgram/ml to 2560 microgram/ml for all examined strains except for 1 strain of S. intermedius of 320 microgram/ml. The examined extracts revealed a cytotoxic effect on the VERO cell line in concentrations higher than 40 microgram/ml.
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