In vitro bulblet regeneration from immature embryos of endangered and endemic Muscari azureum
2011
Uranbey, S., University of Çankırı Karatekin, Çankiri (Turkey). Faculty of Sciences, Department of Biology
A high frequency of bulblet regeneration was achieved for the endemic and endangered ornamental plant Muscari azureum using immature embryos. Immature embryos of M. azureum were cultured on a callus induction medium consisting of N6 mineral salts and vitamins, 400 g/L casein + 40 g/L sucrose + 2 mg/L L-proline, 2 mg/L 2,4-D and 2 g/L Gelrite. Then the embryogenic callus clusters were transferred to a bulblet induction medium consisting of MS mineral salts and vitamins containing different concentrations and combinations of BAP, KIN, TDZ, Zeatin, IAA, NAA, 30 g/L sucrose and 7 g/L agar. Prolific bulblet multiplication (over 13 bulblets/embryo) was achieved from immature embryos after 5-6 months of culture initiation. Well-developed bulblets were excised and individually rooted on 1/2 strength MS medium supplemented with 1 mg/L IBA, 0.5 g/L activated charcoal, 20 g/L sucrose and 6 g/L agar and acclimatized.
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