Role of the Harderian gland in immune response of chickens with maternal immunity to the vaccine against infectious bronchitis virus. [M.Sc. thesis] | Uloga Harderove žlezde u imunom odgovoru pilića sa maternalnim imunitetom na vakcinu protiv virusnog infektivnog bronhitisa. Magistarska teza
2010
Spalević, Lj.
The Harderian gland in the fowl is a tubuloalveolar gland located medial posterior to the eyeball and its duct opens on the conjunctival sac deep into nictitating membrane naso-ventrally. It has two important functions: production of a secretion that lubricates the nictitating membrane and protection of the oculonasal region from exogenous antigens. The aim of this study was to prove the role of the Harderian gland as an immune organ when vaccinating broiler chickens against infections bronchitis and Newcastle disease and also that maternal antibodies to IBV have no impact on the vaccination of one day old chikens with attenuated H120 infectious bronchitis virus vaccine strain. The investigated chickens were divided into three groups. One was used as a control and was not immunized. Experimental group O1 was immunized by the method of spray on the first day of age against infectious bronchitis vaccine H120 and experimental group O2 was immunized on the first day as the previous one but on the 11th day it received a vaccine against Newcastle disease with La Sota strain. The experiment lasted 21 days. Blood sera were tested for the presence of specific antibodies to IBV by ELISA test and the NDV Hi test. We observed the histological material Harder gland and the appearance of plasma cells using microscopy. The obtained results confirm that the Harderian gland has the function of secondary lymphoid organ and that maternal antibodies do not have influence on the immune response of Harderian gland.
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